Cholesterol oxidase(COD/CHOD)
Whakaahuatanga
Ko te Cholesterol oxidase (CHOD) e whakakorikori ana i te mahi tuatahi mo te catabolism cholesterol.Ko etahi huakita kore-pathogenic, penei i a Streptomyces ka taea te whakamahi cholesterol hei puna waro.Ko nga huakita pathogenic, penei i te Rhodococcus equi, ka hiahia a CHOD ki te whakakino i te macrophage o te kaihautu. Ko te CHOD he mahi takirua. Ko te Cholesterol i te tuatahi ka waikura ki te cholest-5-en-3-one i roto i te waahanga e hiahiatia ana e te FAD.Ko te cholest-5-en-3-one kua whakawehea ki te cholest-4-en3-one. Ko te tauhohenga whakawehenga ka taea te huri i tetahi waahanga.Ko te ngohe o te CHOD e whakawhirinaki ana ki nga ahuatanga o te kiriuhi e herea ai te tïpako.
Ka whakamahia te CHOD hei whakatau i te cholesterol serum.Koia te tuarua e whakamahia nuitia ana i roto i nga tono tātaritanga whai muri i te glucose oxidase.Ka kitea hoki e te CHOD te tono i roto i te microanalysis o te steroids i roto i nga tauira kai me te wehewehe i te 3-ketosteroids mai i te 3b-hydroxysteroids. Kei te tirotirohia nga tipu whakawhiti e whakaatu ana i te cholesterol oxidase i roto i te whawhai ki te pepeke miro.Ko te Cholesterol oxidase kua whakamahia ano he rangahau rapoi hei whakamaarama i nga hanganga membrane pūtau.
Hanganga matū
Kaupapa Tauhohenga
Cholesterol + O2 →△4-Cholesten-3-one + H2O2
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | Te paura amorphous kowhai, kua lyophilized |
| Mahi | ≥8U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa (10mg paura/ml) | Maamaa |
| Catalase | ≤0.001% |
| Te huka oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Te kawe me te rokiroki
Te kawe waka:Ka tukuna raro -15°C
Rokiroki :Rokiroki ki te -25~-15°C(Wā roa), 2-8°C(Wā poto)
Ka tūtohuhia te whakamatautau anoTe ora:1 tau
