D-Lactate dehydrogenase(LDH)
Whakaahuatanga
Ka whakamahia te enzyme mo te tango i te pyruvate i roto i nga whakatau e mahi ana me te NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Whakamahinga
1.Ka taea e te phosphatase Alkaline honoa ki te pūmua (antibody, streptavidin etc,) te tautuhi motuhake i nga ngota ngota, a ka taea te whakamahi i roto i te ELISA, WB me te kitenga matū;
2.Ka taea te whakamahi i te phosphatase alkaline ki te whakakore i te 5 '-terminal o te DNA, te RNA ranei hei aukati i te hono-whaiaro;
3. Ko te DNA dephosphorylated, RNA ranei i runga ake nei ka taea te tapaina e nga phosphates tapanga-irirangi (ma te T4 poly-nucleotide kinase)
Hanganga matū
Tikanga tauhohenga
D-Lactate + NAD+→ Pyruvate +NADH + H+
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | Te paura amorphous ma, kua lyophilized |
| Mahi | ≥200U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa (10mg paura/mL) | Maamaa |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Te kawe me te rokiroki
Te kawe waka:Ambient
Rokiroki :Rokiroki ki te -20°C(Waa roa), 2-8°C (waa poto)
Ka tūtohuhia te whakamatautau anoTe ora:2 tau
