Peroxidase (Mapuna Horseradish) He kupu taurite: Hydrogen peroxide oxidoreductase;HRP

Ko te Horseradish peroxidase (HRP) ka wehea mai i nga pakiaka o te horseradish (Amoracia rusticana) a no te roopu ferroprotoporphyrin o peroxidases.Ka hono noa a HRP ki te hauwai peroxide (H2O2).Ko te huanga [HRP-H2O2] matatini ka taea te whakapouri i te maha o nga momo koha hauwai:
Kaituku + H2O2 → Kaituku Oxidized + 2 H2O
Ka whakawaia e te HRP nga momo substrate (tirohia te Ripanga 1):
• Chromogenic
• Maama (pērā i te luminol me te isoluminol)
• Fluorogenic (pērā i te tyramine, te waikawa homovanillic, te waikawa 4-hydroxyphenyl acetic ranei)
Ko te HRP he polypeptide mekameka kotahi kei roto nga piriti disulfide e wha.Ko te HRP he glycoprotein he 18% te warowaihā.Ko te hanganga warowaiha he galactose, arabinose, xylose, fucose, mannose, mannosamine, me te galactosamine, i runga i te isozyme motuhake.

Ko te HRP he tapanga e whakamahia nuitia ana mo nga immunoglobulins i roto i te maha o nga momo tono maakuta mate, tae atu ki te immunoblotting, immunohistochemistry, me te ELISA.Ka taea te hono te HRP ki nga antibodies ma te maha o nga tikanga rereke, tae atu ki te glutaraldehyde, te waiora o te wa, na roto i nga here disulfide, me nga hononga-whakawhitinga a amino me te tiol.Ko te HRP te tapanga e tino hiahiatia ana mo nga antibodies, na te mea ko te mea iti rawa me te tino pumau o nga tapanga whaariki e toru e tino rongonui ana (peroxidase, β-galactosidase, alkaline phosphatase) me tana glycosylation ka heke te herenga kore-motuhake.Kua whakaputaina he arotake mo te glutaraldehyde me nga tikanga whakakotahi i te waa.

Ka whakamahia ano te Peroxidase mo te whakatau i te glucose4 me te peroxides i roto i te otinga.He maha nga whakaputanga, 6-24 tuhinga, 25-29 me nga tuhinga roa 30-46 kua whakahua i te whakamahinga o te P8375 i roto i o raatau tikanga rangahau.