Uricase(UA-R) mai i te Microorganism
Whakaahuatanga
He pai tenei enzyme mo te whakatau i te waikawa uric i roto i te tātari haumanu.Ka uru a Uricase ki te purine catabolism.Ka whakakorikori i te huringa o te waikawa uric tino kore e rewa ki te 5-hydroxyisourate.Ko te whakaemi o te waikawa uric ka mate te ate, te whatukuhu, te mate kauti ranei.I roto i nga kiore, na te huringa o te ira e whakawaehere ana i te uricase ka piki ohorere te waikawa uric.Ko nga kiore, he ngoikore ki tenei ira, ka whakaatu i te hyperuricemia, te hyperuricosuria, me te nephropathy aukati tioata waikawa uric.
Hanganga matū
Kaupapa Tauhohenga
Te waikawa uric+O2+2H2O→ Allantoin + CO2+ H2O2
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | Te paura amorphous ma, kua lyophilized |
| Mahi | ≥20U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa (10mg paura/ml) | Maamaa |
| Kei roto i nga enzymes | |
| NADH/NADPH oxidase | ≤0.01% |
| Catalase | ≤0.03% |
Te kawe me te rokiroki
Te kawe waka:Ka tukuna i raro i te -20°C
Rokiroki :Rokiroki ki te -20°C(Wā roa), 2-8°C(Wa poto)
Ka tūtohuhia te whakamatautau anoTe ora:2 tau
hua e pa ana
Tuhia to korero ki konei ka tukuna mai ki a matou
