Glucose-6-phosphate dehydrogenase (G6PDH)
Whakaahuatanga
Ko te ngoikoretanga o te Glucose 6 phosphate dehydrogenase (G6PD) he mate tuku iho e pakaru ai nga toto toto whero (hemolysis) ina pa ana te tinana ki etahi kai, raau taero, mate, ahotea ranei.Ka puta ina ngaro te tangata, he iti ranei nga taumata o te enzyme glucose-6-phosphate dehydrogenase.Ko tenei enzyme ka awhina i nga kamera toto whero ki te mahi tika.Ko nga tohu i te wa o te wahanga hemolytic ko te mimi pouri, te ngenge, te koma, te tere o te ngakau, te poto o te manawa, me te kowhai o te kiri (jaundice).Ko te ngoikoretanga o te G6PD he mea tuku iho i roto i te hononga hono-X, he maha ake nga tohu i roto i nga tane (ina koa nga tangata o Awherika o Awherika me era o etahi waahi o Awherika, Ahia, me te Mediterranean).Ka puta mai i nga huringa ira i roto i te ira G6PD.
Ko te Glucose-6-Phophate Dehydrogenase (G-6-PDH) e rua nga waahanga iti o te taumaha ngota rite. Kua whakaputaina te raupapa waikawa amino o te monomer. Kua whakamahia te G-6-PDH ki nga whakamatautau mo te nicotinamide adenine dinucleotide me te pyridine kiko. nucleotides.Ko te G-6-PDH ka taea te whakahohe mai i nga otinga kua whakahekehia te Urea.
Ko te Glucose 6-phosphate dehydrogenase he whākōkī ture matua i te taahiraa tuatahi o te ara pentose phosphate.Ko te G-6-P-DH ka whakahāorahia te glucose-6- phosphate i te aroaro o te NADP+ ki te tuku 6- phosphogluconate.Ko te electrophoresis gel polyacrylamide, te whakapouri mahi, me nga rangahau antibody antibody G-6-PDH antibody immunoblotting kua tohu ko te G-6-PDH he glycoprotein.
Hanganga matū
Kaupapa Tauhohenga
D-Glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | Te paura amorphous ma, kua lyophilized |
| Mahi | ≥150U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa (10mg paura/ml) | Maamaa |
| NADH/NADPH oxidase | ≤0.1% |
| Glutathione reductase | ≤0.001% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Te kawe me te rokiroki
Te kawe waka: Ambient
Rokiroki :Rokiroki ki te 2-8°C
Ka tūtohuhia te whakamatautau anoTe ora:2 tau
