Fructosyl-peptide Oxidase (FPOX)
Whakaahuatanga
He whai hua te enzyme mo te whakatau i te fructosyl-peptide me te waikawa fructosyl-L-amino.
Hanganga matū
Kaupapa Tauhohenga
Fructosyl-peptide + H2O + O2→ Peptide + Glucosone + H2O2
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | Te paura amorphous ma, kua lyophilized |
| Mahi | ≥4U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Catalase | ≤0.01% |
| ATPase | ≤0.005% |
| Te huka oxidase | ≤0.03% |
| Cholesterol oxidase | ≤0.003% |
Te kawe me te rokiroki
Te kawe waka: Ambient
Rokiroki:Rokiroki ki te -20°C(Waa roa), 2-8°C (waa poto)
Ka tūtohuhia te whakamatautau anoTe ora:2 tau
Hītori whanaketanga
Ko tetahi o nga tohu e whakamahia ana mo te mate huka ko te hemoglobin glycated (HbA1c).Ko te inenga o te HbA1c ma te whakamahi i nga enzyme e tika ana mo te tukatuka i te maha o nga tauira, he pai te utu.Na tenei, kua roa te karanga a nga tohunga hauora mo te whakawhanaketanga o taua momo whakamatautau whaa.Na reira, i whakawhanakehia e matou he whakamatautau hou ma te whakamahi i te "tikanga dipeptide".Inaa, i kitea e matou ko "Fructosyl-peptide Oxidase" (FPOX) ka taea te whakamahi hei whaawha mo tenei whakamatautau.Na tenei i angitū to maatau ki te eke ki te taumata tuatahi o te ao ma te mahi pono o te whakamatautau HbA1c enzyme.Ko tenei "tikanga dipeptide" ka whakamahi i te Protease (Proteolytic enzyme) ki te wawahi i te HbA1c i roto i te rerenga toto, ka ine i nga taumata o nga dipeptides saccharified i hangaia ma te FPOX.I tutuki tenei tikanga me te tino pai o te manaakitanga na te mea he ngawari, he iti te utu me te tere, a kua tae mai te reagent ine HbA1c ma te FPOX ki te whakamahi puta noa i te ao.
