Glucose Dehydrogenase(GDH)
Whakaahuatanga
Ko te Glutamate Dehydrogenase (GDH) he whākōkī mitochondrial e whakakorikori ana i te whakakorenga oxidative reversible o te glutamate ki te a-ketoglutarate me te mahi hei hononga matua i waenga i nga ara anabolic me te catabolic.I roto i nga kararehe whakangote, ko te GDH kei raro i te ture allosteric me te mahi nui i roto i te ate, te whatukuhu, te roro, me te pancreas.Ka taea te whakamahi i te mahi GDH i roto i te serum ki te wehewehe i waenga i nga mate ate na te mumura o te ate, kaore e whakaatu i te mahi GDH serum teitei, me nga mate e hua ana i te necrosis hepatocyte, e hua ana te GDH serum teitei.
Ko te mahi a te GDH ka whakatauhia e te whakahiato enamaki e hono ana ka pau te glutamate e te GDH whakangao NADH, ka tauhohe me te rangahau e whakaputa hua tae (450 nm) e rite ana ki te ngohe GDH kei reira.Kotahi te waeine o te GDH ko te nui o te whākōkī ka whakaputa i te 1.0 mmole o te NADH ia meneti i te pH 7.6 i te 37 °C
Hanganga matū
Tikanga tauhohenga
D-Glucose + kaikawe → D-Glucono-1,5-lactone + te kaikawe whakaheke
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | Te paura amorphous ma, kua lyophilized |
| Mahi | ≥160U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa(10 mg paura/mL) | Maamaa |
| Whakakino i nga enzymes | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Te kawe me te rokiroki
Te kawe waka: Puka tio
Rokiroki :Rokiroki ki te -25~-15°C(Wā roa), 2-8°C (wā poto)
Ka tūtohuhia te whakamatautau anoTe ora: 18 marama
