Ultra Nuclease-mRNA Synthesis Raw Material Whakaahua Whakaaturanga

Nuclease Ultra

Nama nama:9025-65-4

EC.Nama: 3.1.30.2

Mōkī:20μL, 200μL,2mL, 20mL

Tuku Uiui

Taipitopito Hua

Whakaahuatanga

Ko te UltraNuclease he endonuclaese hangaia ira i ahu mai i te Serratia marcescens, e kaha ana ki te whakaheke i te DNA, i te RNA ranei, te paerua, te pae kotahi ranei, te raina, te porohita ranei i raro i te whānuitanga o nga ahuatanga, ka tino whakaheke i nga waikawa karihi ki te 5'-monophosphate oligonucleotides me te 3-5 roa turanga.

I muri i te whakarerekētanga ira ira, i fermented te hua, te whakapuaki, ka purea i roto i te Escherichia coli (E. coli), e whakaiti i te pokey o supernatant pūtau me te lysate pūtau i roto i te rangahau pūtaiao, engari ano te whakapai ake i te tōtika purenga me te rangahau mahi o te pūmua.Ka taea hoki te whakamahi i roto i te rongoa ira, te purenga huaketo, te hanga kano kano, te pūmua me te ahumahi rongoa polysaccharide hei reagent tango waikawa karihi kaihautu.

Hanganga matū

Whakamaramatanga Waeine

Ko te nui o te whākōkī i whakamahia ki te huri i te uara absorption o △A260 mā te 1.0 i roto i te 30min i te 37 °C, pH 8.0, he rite ki te 37μg salmon sperm DNA i kerihia ma te tapahi ki te oligonucleotides, i tautuhia hei waeine kaha.

Te Whakamahi me te Inenga

• Tangohia te waikawa karihi o waho mai i nga hua kano kano, whakaiti i te tupono o te toenga o te paitini waikawa karihi me te whakapai ake i te haumaru hua.
• Whakaitihia te pokey o te wai whangai na te waikawa karihi, whakapoto i te wa tukatuka me te whakanui ake i te hua o te pūmua.
• Tangohia te waikawa nucleic i takai matūriki (huaketo, tinana whakauru, aha atu), e pai ana ki te tuku me te purenga o te matūriki.
• Ka taea e te maimoatanga karihi te whakapai ake i te whakatau me te whakaora i te tauira mo te chromatography tīwae, electrophoresis me te tātari blotting.
• I roto i te whakamaarama ira, ka tangohia te waikawa karihi ki te whiwhi i nga huaketo adeno-hononga.

Whakatakotoranga

Nga Tuemi Whakamatau	Whakatakotoranga
Whakaahuatanga	Maama me te Taekore
Mahi	≥ 250 U/ul
Mahi motuhake	≥1.1*10⁶U/mg
Pure （SDS-WAHI）	≥ 99.0%
Porotei	Karekau i kitea
Koiora	＜10 cfu/100,000U
Endotoxine(whakamatautau-LAL)	＜ 0.25EU/1,000U