Ko te Kete Whakamatau Nuclease Ultra (ELISA) Atahanga Whakaatu

Kete Whakatau Nuclease Ultra(ELISA)

Nama nama:9025-65-4

EC.Nama: 3.1.30.2

Mōkī: 96 Whakamātautau

Tuku Uiui

Taipitopito Hua

Whakaahuatanga

Ko tenei kete Ultra Nuclease ELISA he onepu ka mahia a ELISA ki te whakatakotoranga moroiti.Ko te tauira kei roto pea te endonuclease kei roto i nga puna pereti microtiter kua pania-mua ki te parepare hopu anti-endonuclease kua purea.Whai muri i te hekitanga me te horoi horoi ka tangohia nga waahanga kore i herea, ka taapirihia he anti-endonuclease detector antibody.Ko nga tauhohenga mate mate ka puta he hangahanga o te hanawiti o te wahanga totoka antibody-endonuclease-enzyme kua tapaina he antibody.Whai muri i te horoinga whakamutunga, ka taapirihia he otinga tïpako ki nga puna ka tauhohe, ka puta he tae.Ko te kiato whatu ka ine whakaahua me te orite ki te
te kukū wetewete kei roto i nga puna.Ko te kukū o te endonuclease i roto i nga tauira e kore e mohiotia ka taea te tatau i runga i te pihi paerewa e rite ana.

Hanganga matū

Whakamaramatanga Waeine

Te nui o te whākōkī e whakamahia ana hei huri i te uara whakauru o △A260 mā te 1.0 i roto i te 30meneti
i te 37 °C, te pH 8.0, he rite ki te 37μg salmon sperm DNA sperm digested ma te tapahi ki te oligonucleotides, i tautuhia hei waeine kaha.

Te Whakamahi me te Inenga

• Tangohia te waikawa karihi o waho mai i nga hua kano kano, whakaiti i te tupono o te toenga o te paitini waikawa karihi me te whakapai ake i te haumaru hua.
• Whakaitihia te pokey o te wai whangai na te waikawa karihi, whakapoto i te wa tukatuka me te whakanui ake i te hua o te pūmua.
• Tangohia te waikawa nucleic i takai matūriki (huaketo, tinana whakauru, aha atu), e pai ana ki te tuku me te purenga o te matūriki.
• Ka taea e te maimoatanga karihi te whakapai ake i te whakatau me te whakaora i te tauira mo te chromatography tīwae, electrophoresis me te tātari blotting.
• I roto i te whakamaarama ira, ka tangohia te waikawa karihi ki te whiwhi i nga huaketo adeno-hononga.