BspQI Restriction Endonuclease

Nama cas:81295-42-3

Mōkī: 50μL, 250μL, 1ml,10ml, 100ml

Tuku Uiui

Taipitopito Hua

Whakaahuatanga

Ko te BspQI ka taea te whakaatu ano i roto i te E. coli e mohio ana ki nga waahi motuhake ka hangaia i raro i nga paerewa GMP.Kei te whakamahia nuitia i roto i te mapi ira, te cloning me etahi atu mara.He tere te tapahi i te DNA mo te whakariterite ira ira.
5'······GGTCTC(N)1······3'
3'······CCAGAG(N)4······5'

Hanganga matū

Whakamaramatanga Waeine

Kotahi te waeine kua tautuhia ko te nui o te whākōkī e hiahiatia ana ki te keri i te 1 µg o te λ DNA i roto i te 1 haora i te 50°C i roto i te tapeke o te rōrahi tauhohenga o te 50 µl.

Nga waahanga hua

• He nui te mahi enzymatic o te endonuclease here, ka oti te nakunaku.
• He iti te mahi endonuclease kore-motuhake hei whakarite i te tapahi "scalpel" tika.
• Kaore he punaha BSA, ka whakaiti i te parahanga o te wera.

Whakatakotoranga

Nga Tuemi Whakamatau	Whakatakotoranga
Pūmua Pure	＞98%
Te toenga o te Protease	Karekau i kitea
Te hononga me te kitenga tapahi	He pai te nakunaku>>95%
DNAase	Karekau i kitea
Nicaknase	Karekau i kitea