Kete whakamatautau RNase (Fluorescence)
Whakaahuatanga
Ko te kete rapunga RNase kei runga i te rangahau RNA tapanga-fluorophore, ka taea e nga kairangahau te ine i te mahi RNase.Mena karekau he mahi RNase i roto i te tauira, ka noho pumau te rangahau, karekau hoki e puta he tohu korikori;ina kei roto i te tauira te mahi RNase, ka whakahekehia te rangahau, ka puta te tohu o te tohu rewharewha;Ko te tere o te pikinga o te tohu rewharewha he pai te hono ki te maha me te ngohe o nga enzymes.
Hanganga matū
Te titonga kete
Ingoa | 48Whakamatau | 192Whakamatau |
10 × otinga tauhohenga | 0.5mL | 2.0mL |
RNA rangahau | 1 ngongo | 1 ngongo |
TE parepare | 0.5mL | 2.0mL |
Paerewa RNase A (10mg/mL) | 10μL | 20μL |
Paerewa Dilution Buffer | 6mL | 12mL |
DNase&RNase-kore wai | 25mL | 25mL |
DNase RNase atu | 50mL | 50mL |
Whakatakotoranga
Nga Tuemi Whakamatau | Whakatakotoranga |
Te ahua | Kua oti te whakakii me te kore e pakaru te wai |
Tepe rapunga | RNase A: 0.313pg/mL |
Tikanga | Whakamaarotoroto CV ≤ 10% |
Te kawe me te rokiroki
Te waka:Te hukapapa maroke
Rokiroki:Rokiroki ki te -20°C(kaore i te whakamau me te whakamaroke tonu)
E taunaki ana kia whakamatauria ano te Ora:1 tau
hua e pa ana
Tuhia to korero ki konei ka tukuna mai ki a matou