Phosphatase Alkaline(ALP)

Ko te Phosphatase Alkaline i ahu mai i te riaka E. coli recombinant e kawe ana i te ira TAB5.Ka whakakorikori te enzyme i te whakakorenga o te 5' me te 3' pito o te DNA me te RNA phosphomonoesters.I tua atu, ka whakahekehia te ribose, me te deoxyribonucleoside triphosphates (NTPs me dNTPs).TAB5 Alkaline Phosphatase e mahi ana i runga i te 5' e puta mai ana, e 5' nga pito karekau me nga pito puhuki.Ka taea te whakamahi i te Phosphatase i roto i te maha o nga tono koiora irapoi, penei i te whakakao, i te tapanga mutunga tirotiro ranei hei tango i nga pito phosphorylated o DNA, RNA ranei.I roto i nga whakamatautau whakamaarama, ka aukati te dephosphorylation i te DNA plasmid raina mai i te whakakotahitanga whaiaro.Ka taea ano e ia te whakaheke i nga dNTP kaore i whakauruhia ki nga tauhohenga PCR hei whakarite i tetahi tauira mo te raupapa DNA.Ko te whākōkī e kore e taea te whakahoaho mā te whakamahana i te 70°C mō te 5 meneti, nā reira ka kore e tika te tango i te phosphatase i mua i te herenga, te tapanga mutunga ranei.