Deoxyribonuclease I(Dnase I)
Whakaahuatanga
Ko te DNase I (Deoxyribonuclease I) he endodeoxyribonuclease ka taea te keri i te DNA whenu kotahi, takirua ranei.Ka mohio, ka wehe i nga here phosphodiester ki te whakaputa i te monodeoxynucleotides, oligodeoxynucleotides kotahi, takirua ranei me nga roopu phosphate i te 5'-terminal me te hydroxyl i te 3'-terminal.Ko te ngohe o te DNase I e whakawhirinaki ana ki te Ca 2+ ka taea te whakahohe e nga katote whakarewa divalent penei Mn 2+ me Zn 2+.Ko te 5 mM Ca 2+ he tiaki i te whākōkī mai i te hauwai.I te aroaro o Mg 2+ , ka taea e te enzyme te mohio matapōkeretia me te wawahi i tetahi waahi i runga i tetahi whenu DNA.I te aroaro o Mn 2+ , ka taea te mohio nga whenu takirua o te DNA i te wa kotahi ka wehea ki te tata ki te waahi kotahi ki te hanga i nga kongakonga DNA mutunga papatahi, nga kongakonga DNA mutunga piri me te 1-2 nucleotides e puta ana.
Hanganga matū
Whakamaramatanga Waeine
Kotahi te waeine kua tautuhia ko te nui o te whaakiki ka tino whakaheke i te 1 µg o pBR322 DNA i roto i te 10 meneti i te 37°C.
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Pure (SDS-PAGE) | ≥ 95% |
| Mahi Rnase | Kore Whakahekea |
| gDNA Contamination | ≤ 1 kape/μL |
Te kawe me te rokiroki
Te waka:Ka tukuna i raro i te 0 °C
Rokiroki:Rokiroki i te -25~-15°C
E taunaki ana kia whakamatauria ano te Ora:2 tau
