BsaI Restriction Endonuclease

Maamaa:20U/μL

Mōkī: 50μL, 250μL, 1ml,10ml, 100ml

Tuku Uiui

Taipitopito Hua

Whakaahuatanga

Ko te whākōkī aukati BsaI he momo II herenga endonuclease i ahu mai i te pūmua recombinant kua whakawaeheretia e te ira BspQI i roto i te Bacillus sphaericus i whakaatuhia e E.coli.Ka whakamahia nuitia nga endonucleases here i roto i te mapi ira, te cloning me etahi atu mara.Ka tere te tapahi i te DNA mo te whakahiato ira ira Bsa I , he IIs restriction endonuclease restriction endonuclease, i ahu mai i te riaka E. coli recombinant e kawe ana i te ira BsaI kua whakakohia me te whakarereke mai i Bacillus stearothermophilus.he rite tonu te ahua ki nga enzymes taketake me te iti o te mahi whetu.Ko te raupapa tohu me nga waahi wehewehe e whai ake nei:

5'·····GGTCTC(N)······ ··3'

3'······CCAGAG(NNNNNN)······5'

Hanganga matū

āhuatanga hua

• He nui te mahi enzymatic o te endonuclease here, a, ka oti te hohenga nakunaku.

• He iti te mahi endonuclease kore-motuhake hei whakarite i te tapahi "scalpel" tika.

• Kaore he punaha BSA, ka whakaiti i te parahanga o te wera.

Whakatakotoranga

Nga Tuemi Whakamatau	Whakatakotoranga
Mahi Enzyme	20 U/µL
Pure(SDS-PAGE)	≥95%
Rnase	Karekau i kitea
Nga Mahi DNase Kore-motuhake	Kaore i kitea
Nga Mahi Whetu	Kaore i kitea
Te Whakapiri me te Recutting	I muri i te nakunakutanga, ka taea te hono nga kongakonga DNA.A ko enei kongakonga hono ka taea te tapahi ki te Bsal.
Endotoxins(whakamatautau-LAL)	≤10 EU/mg
E.Coli DNA	≤1 Tārua/2U

Mana kounga

Purity ≥ 95%, No DNase, RNase mahi, Host DNA toenga ≤ 100pg/mg, toenga pūmua ope ≤ -50ppm, toenga endotoxin ≤ 10EU/mg, Kaore he mahi protease, sterile, kore he mycoplasma

Taupānga

Kena te plasmid ki te whakarite i te wahanga DNA kua whakararangitia i te pito o te Poly (A/T/C/G);

Whakaritehia te tauira plasmid i mua i te tuhi in vitro;

Te kemu here;

Whakamaramatanga Waeine

Kotahi te waeine kua tautuhia ko te nui o te whākōkī e hiahiatia ana ki te keri i te 1 µg o te DNA Mana-roto i roto i te 1 haora i te 37°C i roto i te rahinga tauhohenga katoa o te 50 µL