T4 RNA here
Whakaahuatanga
Ko tenei hua ko te T4 RNA ligase (T4 RNA Ligase I) e whakawhirinaki ana ki te ATP e whakaatuhia ana e E. coli.Ko tenei enzyme ka whakakorikori i te hanganga o nga here phosphodiester i waenga i te oligonucleotides, te RNA tautahi me te DNA intermolecular/ intra-molecular 5'-PO4 me te 3'-OH.
Whakamaramatanga Waeine
Kotahi te waeine kua tautuhia ko te nui o te whaakiki e hiahiatia ana hei huri i te 1 nanomole o te 5'-[³²P] rA16 ki te ahua phosphatase-resistant i roto i te 30 meneti i te 37°C.
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Mahi Enzyme | 10 U/μL |
| Mahi Pūmua | ≥ 95% |
| Nickase | Kaore e kitea |
| Mahi RNase | Kaore e kitea |
| Ecoli gDNA | ≤1 kape/50U |
| Endotoxin | LCL-Whakamātautau,≤10EU/mg |
Te kawe me te rokiroki
Te waka:Te hukapapa maroke
Rokiroki:Rokiroki ki te -25~-15°C(kaore i te tio me te whakamaroke tonu)
E taunaki ana kia whakamatauria ano te Ora:2 tau
hua e pa ana
Tuhia to korero ki konei ka tukuna mai ki a matou
