ELEISA KIT mo Trypsin

He maha nga wa e whakamahia ana a Trypsin Recombinant i roto i nga mahi whakangao koiora-i te wa e whakarite ana i nga pūtau, mo te whakarereketanga me te whakahohe i nga hua.Ko te Trypsin he raru mo te haumaru, na reira me tango i mua i te tukunga o nga hua whakamutunga.Ko tenei Kete Sandwich mo te inenga ine o te toenga Trypsin i roto i te supernatant ahurea pūtau me etahi atu tikanga i roto i te hanga rongoa koiora ina whakamahia ana a Trypsin.

Ko tenei kete he Enzyme-Linked Immunosorbent Assay (ELISA).Ko te pereti kua pania-mua ki te Porcine trypsin antibody.Ko te trypsin kei roto i te tauira ka taapirihia ka herea ki nga paturopi kua pania ki nga puna.Na ka taapirihia te antibody Porcine trypsin biotinylated ka herea ki te trypsin i roto i te tauira.Whai muri i te horoinga, ka taapirihia te HRP-Streptavidin ka herea ki te antibody trypsin Biotinylated.I muri i te hekitanga ka horoia te HRP-Streptavidin.Katahi ka taapirihia te otinga tïpako TMB ka whakakorikorihia e te HRP kia puta he hua tae kahurangi ka huri ki te kowhai i muri i te taapiri i te otinga aukati waikawa.Ko te kiato o te kowhai he rite ki te nui o te trypsin
tauira ka mau ki te pereti.Ka inehia te urunga ki te 450 nm.