Ribonuclease III (RNase III)

EC 3.1.24

Pure.: 2U/μL

Mōkī: 50μL,125μL

Tuku Uiui

Taipitopito Hua

Whakaahuatanga

Ko tenei hua ko te ribonuclease III (RNase III) e whakaatuhia ana e E. coli.Ko tenei exonuclease motuhake ka tihorea te RNA (dsRNA) taurua, ka whakaputa i nga kongakonga dsRNA 12-35bp me te 5'-PO4 me te 3'-OH, 3' te whakairinga.

Hanganga matū

Whakamaramatanga Waeine

Te whakamaramatanga o te wae mahi: Kotahi te waeine e tohu ana ki te nui o te whākōkī e hiahiatia ana hei whakaheke i te 1 μg o
dsRNA ki te siRNA i roto i te punaha tauhohenga 50 μL i te 37°C mo te 20 meneti.

Whakatakotoranga

Nga Tuemi Whakamatau	Whakatakotoranga
Mahi Exonuclease	Nga Putanga< 0.1% o te tapeke irirangi
Nga Mahi Dnase Kore-motuhake	Kaore e kitea
Whakamātautau Maama Pūmua(SDS-PAGE)	≥ 95%
Mahi RNase (Te Kenanga Atu)	＞90% o te RNA tïpako kei te mau tonu