Ribonuclease A (RNase A)

Ko te Ribonuclease A (RNase A) he polypeptide-kotahi kei roto e 4 nga here disulfide me te taumaha ngota o te 13.7 kDa.Ko te RNase A he endoribonuclease e tino whakaheke ana i te RNA whenu kotahi ki nga toenga C me U.Ko te mea motuhake, ka mohio te wehenga ki te here phosphodiester i hangaia e te 5'-ribose o te nucleotide me te roopu phosphate i runga i te 3'-ribose o te pirimidine nucleotide e tata ana, na te 2', 3' - Cyclic phosphates e hydrolyzed ki te rite. 3'-nucleoside phosphates (hei tauira, pG-pG-pC-pA-pG ka wehea e RNase A hei whakaputa pG-pG-pCp me A-PG).Ko te RNase A te mea tino kaha ki te wawahi i te RNA tauhohenga kotahi me te kaha i roto i nga momo ahuatanga tauhohenga: i te iti o te tote (0 ki te 100 mM NaCl), ka taea te whakamahi ki te wawahi i te RNA-tahi, te RNA-rua, me nga whenu RNA i roto i nga ranu RNA-DNA.i te nui o te tote tote (≥0.3 M), ka taea e te RNase A te wehe motuhake te RNA whenu kotahi.