Kotahi Hipanga RT-qPCR kete tirotiro
Whakaahuatanga
U+ One Step RT-qPCR Probe Kit (Glycerol-free) he glycerol-free one-step RT-qPCR reagent e whakamahi ana i te RNA hei tauira (penei i te huaketo RNA), e tika ana mo te whanaketanga me te hoahoa o nga hua lyophilized.Ko tenei hua e whakauru ana i nga mahi pai ake o te Reverse Transcriptase kotahi-taahiraa me te Champagne Taq DNA Polymerase, me te whakamaroke matū whakamaroke whakatio pai, he pai ake te kaha o te whakakaha, te toenga me te motuhake, a he hototahi ki nga momo whakamaroke-tio. I tua atu, ka whakauruhia te punaha anti-pollution dUTP / UDG ki roto i te reagent, ka taea te mahi i te pāmahana o te rūma, te whakakore i te paanga o te whakapouritanga hua whakakaha i runga i te qPCR, me te whakarite i te tika o nga hua.
Nga Tikanga Taketake o RT-qPCR
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga | Hua |
| (SDS PAGE) Pure o te Hoa Enzyme(SDS PAGE) | ≥95% | Paahi |
| Mahi Endonuclease | Kaore i kitea | Paahi |
| Mahi Exodulease | Kaore i kitea | Paahi |
| Mahi Rnase | Kaore i kitea | Paahi |
| Te toenga E.coli DNA | ＜1 kape/60 | Paahi |
| Pūnaha Whakamātautau Mahi | 90%≤110% | Paahi |
Waehanga
| Waehanga | 100rxns | 1,000rns | 5,000 rxns |
| RNase-kore ddH2O | 2*1ml | 20ml | 100ml |
| 5 * kotahi te ranunga taahiraa | 600μl | 6*1ml | 30ml |
| Kotahi te hikoi te ranunga enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX tohutoro Waikano 1 | 60μl | 600μl | 3*1ml |
| 50* ROX tohutoro Waikano 2 | 60μl | 600μl | 3*1ml |
a.Kotahi-Step Buffer kei roto te dNTP Mix me te Mg2+.
b.Ko te Rahui Enzyme te nuinga he whakamuri
transcriptase, Hot Start Taq DNA polymerase (whakarereke antibody) me te aukati RNase.
c.Ka whakamahia ki te whakatika i te hapa o nga hihi rewharewha i waenga i nga puna rereke.
c.ROX: Me whiriwhiri koe i te whakatikatika kia rite ki te tauira o te taputapu whakamatautau.
Nga tono
Te kitenga QPCR
Te tuku me te rokiroki
Te waka:Puka tio
Nga Tikanga Rokiroki:Rokiroki ki te -20 ℃.
Shief Life:18marama
