dATP/ dCTP/ dGTP/ dTTP/ dUTP (100mM )
Whakaahuatanga
Ko tenei hua he otinga wai kore tae.He pai mo nga momo whakamatautau koiora rapoi tikanga penei i te whakakaha PCR, PCR wa-tūturu, cDNA, te whakahiato DNA noa ranei, te raupapa DNA me te tapanga.Ka taea te waimeha ki te wai ultra-parakore, ka whakatika ki te pH 7.0 me te otinga NaOH parakore teitei, me te ma ≥ 99% (HPLC).I muri i te kitenga, kaore he DNase, RNase me te phosphotase.Ka taea te whakamahi tika i roto i nga momo tauhohenga koiora rauropi tikanga penei i te PCR.
Hanganga matū
Whakaritenga
| Tuemi whakamatautau | Whakatakotoranga |
| Te ahua | He otinga kore tae |
| Te aro | 100mM ±3% |
| Pure (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Mana Kounga1.Karekau he poke o te DNase me te RNase tenei whakaritenga 2. Ko tenei rota o dNTP kua whakamatauria te mahi ki te Taq DNA polymerase me te Pfu DNA polymerase Ko tenei rota o te NTP kua whakamatauria te mahi ki te Q-PCR | |
Nga tono
PCR awhe roa (20 kb)
te whakahiato cDNA me te RT-PCR
PCR wā-tūturu
PCR Paerewa
PCR pono teitei
Te tuku me te rokiroki
Te waka:Puka tio
Nga Tikanga Rokiroki:Rokiroki ki te -25～-15℃
Te ra whakamatautau ano e taunakihia ana:2 tau
Notes
1) Ka taea te rewa i te pāmahana rūma.I muri i te whakakorenga, me penapena ki roto i te pouaka tio, te pati tio ranei.I muri i te whakamahinga, me rongoa ki te -25～-15 ℃ tonu.
2) Mo to haumaru me to hauora, mau koti taiwhanga me nga karapu tuku mo te mahi.
