Uracil DNA Glycoylase (Glycerol-free) Whakaahua Whakaatu

Uracil DNA Glycoylase (Glycerol-kore)

CAS nama: 39369-21-7 EC nama: 3.2.2.27

Mōkī: 100U, 500U,5KU,10KU

Tuku Uiui

Taipitopito Hua

Whakaahuatanga

Ka taea e te UDG Thermosensitive (uracil-DNA glycosylase) te whakakorikori i te hauwai o te turanga uracil o te mekameka DNA kei roto te uracil me te here N-glycosidic o te tuara huka-phosphate hei tuku i te uracil kore utu.Ka whakatauritea ki nga whākōkī UDG noa, ka karohia e nga whākōkī UDG thermosensitive te mahi toenga o nga whākōkī UDG tikanga i muri i te kore whakahohenga, ka taea te whakaheke i nga hua whakakaha dU i te pāmahana rūma.Ka mahi tenei hua i te pāmahana rūma, he tairongo te pāmahana me te ngawari ki te kore mahi.

Hanganga matū

Whakatakotoranga

Te pūmua	Glycosylase
Kaipupuri Hototahi	Pupuri Rokiroki
Te Whakakore i te wera	50°C, 10 min
Whakamaramatanga Waeine	Kotahi te waeine (U) kua tautuhia ko te nui o te whākōkī e hiahiatia ana hei whakakoi i te hauwai o te 1 μg dU-kei roto dsDNA i roto i te 30 meneti i te 25°C.