RT-LAMP Reagent (-20 ℃)
Whakaahuatanga
I tenei wa kei te whakamahia nuitia a LAMP i te hangarau i roto i te mara o te whakakaha hauwaro.Ka whakamahia e 4-6 nga papa tuatahi ka taea te tautuhi i nga rohe motuhake e 6 i runga i te ira i whaaia, me te whakawhirinaki ki te mahi whakaneke whenu kaha o Bst DNA polymerase.He maha nga tikanga kimi LAMP, tae atu ki te tikanga tae, tikanga pH colorimetric, tikanga turbidity, HNB, calcein, aha atu. Ko te RT-LAMP tetahi momo o te tauhohenga LAMP me te RNA hei tauira.Ko te RT-LAMP Kit (UDG plus) kua whakawhanakehia i runga i te tikanga rererangi me te Bst DNA Polymerase, thermotolerant reverse transcriptase, murine RNase inhibitor, me te UDGase.Ko tenei kete LAMP he tino aro nui, he pai te tono tuatahi, me te tino mahi i roto i te motuhake, te manawanui me te pumau.
Waehanga
| 100T | 1000T |
| 600μL | 6mL |
| 100 μL | 1 mL |
1) Kei roto i te Bst Buffer te dN(U)TP me te SYTO-9, me etahi atu, me te 1×Bst Buffer kei roto i te 8 mmol/L Mg2+;
2) Bst Enzyme Mix (UDG plus) kei roto ko UDGase, Reverse Transcriptase, Bst Ⅱ Polymerase, MRI;
Nga tono
Tauhohenga LAMP
Te tuku me te rokiroki
Te waka:Puka tio
Nga Tikanga Rokiroki:Rokiroki ki te -20 ℃
Te ora o te Shief:6 marama
Whakatupato
Mo to haumaru me to hauora, mau koti taiwhanga me nga karapu tuku mo te mahi.
