RT-LAMP Fluorescent Master Mix (Lyophilized Beads)
Whakaahuatanga Hua
I tenei wa kei te whakamahia nuitia a LAMP i te hangarau i roto i te mara o te whakakaha hau werawera.Ka whakamahia e 4-6 nga papaamata ka taea te tautuhi i nga rohe motuhake e 6 i runga i te ira i whaaia, me te whakawhirinaki ki te mahi whakaneke whenu kaha o Bst DNA polymerase.He maha nga tikanga kimi LAMP, tae atu ki te tikanga tae, tikanga pH colorimetric, tikanga turbidity, HNB, calcein, me etahi atu. Ko te RT-LAMP tetahi momo tauhohenga LAMP me te RNA hei tauira.Ko te RT-LAMP Fluorescent Master Mix (Lyophilized Powder) kei roto i te ahua o te Lyophilized Powder, me taapiri noa i nga mea tuatahi me nga tauira ina whakamahia ana.
Whakatakotoranga
Tuemi whakamatautau | Whakatakotoranga |
Endonulease | Kaore i kohia |
Mahi RNase | Karekau i kitea |
Mahi DNAase | Karekau i kitea |
Mahi Nickase | Karekau i kitea |
E. coli.gDNA | ≤10 kape/500U |
Waehanga
Kei roto i tenei hua te Reaction Buffer, RT-Enzymes Mix o Bst DNA Polymerase me Thermotable Reverse Transcriptase, Lyoprotectant me nga Waa Waikano Fluorescent.
Whakanuia
Ko te whakakaha Isothermal o te DNA me te RNA.
Te tuku me te rokiroki
Te waka:Ambient
Nga Tikanga Rokiroki:Rokiroki ki te -20 ℃
Te ra whakamatautau ano e taunakihia ana:18 Marama