Phosphatase Alkaline (ALP)

Nama nama:9001-78-9

EC Nama:3.1.3.1

Mōkī:100μL, 500μL, 10mL, 100mL, 1000mL

Tuku Uiui

Whakaahuatanga Hua

Whakaahuatanga

Ko te Phosphatase Alkaline i ahu mai i te riaka E. coli recombinant e kawe ana i te ira TAB5.Ka whakakorikori te enzyme i te whakakorenga o te 5' me te 3' pito o te DNA me te RNA phosphomonoesters.I tua atu, ka whakahekehia te ribose, me te deoxyribonucleoside triphosphates (NTPs me dNTPs).TAB5 Alkaline Phosphatase e mahi ana i runga i te 5' e puta mai ana, e 5' nga pito karekau me nga pito puhuki.Ka taea te whakamahi i te Phosphatase i roto i te maha o nga tono koiora irapoi, penei i te kati, i te tapanga mutunga tirotiro ranei hei tango i nga pito phosphorylated o DNA, RNA ranei.I roto i nga whakamatautau whakamaarama, ka aukati te dephosphorylation i te DNA plasmid raina mai i te whakakotahitanga whaiaro.Ka taea ano e ia te whakaheke i nga dNTP kaore i whakauruhia ki nga tauhohenga PCR hei whakarite tauira mo te raupapa DNA.Ko te whākōkī e kore e taea te whakahoe mā te whakawera i te 70°C mō te 5 meneti, nā reira ka kore e tika te tango i te phosphatase i mua i te herenga, te tapanga mutunga ranei.

Whakamahinga

1.Ka taea e te phosphatase Alkaline te hono ki nga pūmua (te paturopi, te streptavidin me etahi atu,) ka taea te tautuhi i nga ngota ngota, ka taea te whakamahi i roto i te ELISA, WB me te kitenga histochemical;
2.Ka taea te whakamahi i te phosphatase alkaline ki te whakakore i te 5 '-terminal o te DNA, te RNA ranei hei aukati i te hono-whaiaro;
3.Ko te DNA dephosphorylated, RNA ranei i runga ake nei ka taea te tapanga e nga phosphates tapanga-irirangi (ma te T4 poly-nucleotide kinase)

Hanganga matū

Whakatakotoranga

Nga Tuemi Whakamatau	Whakatakotoranga
Mahi Enzyme	5U/μL
Mahi Endonuclease	Kaore i kitea
Mahi Exonuuclease	Kaore i kitea
Nga Mahi Whakapapa	Kaore i kitea
Mahi RNase	Kaore i kitea
E.coli DNA	≤1 kape/5U
Endotoxin	LAL-Whakamātautau, ≤10EU/mg
Maamaa	≥95%