Kotahi Hipanga RT-qPCR kete tirotiro
Whakaahuatanga
One Step qRT-PCR Probe Kit he mea hanga motuhake mo te qPCR e whakamahi tika ana i te RNA (hei tauira RNA huaketo) hei tauira.Ma te whakamahi i nga ira motuhake (GSP), ka taea te whakaoti i te tuhi whakamuri me te qPCR i roto i te ngongo kotahi, ka tino whakaiti i nga tikanga paipa me te tupono o te poke.Ka taea te whakakore i te 55 ℃ me te kore e pa ki te kaha me te ngawari o te qRT-PCR.Kotahi Hipanga qRT-PCR Probe Kit e whakaratohia ana ki te Master Mix.Kei roto i te 5 × One Step Mix he parepare me te dNTP/dUTP Mix, he pai hoki mo nga punaha rapunga tino-motuhake i runga i nga maataki kua tapaina ki te korikori (hei tauira, TaqMan).
Nga Tikanga Taketake o RT-qPCR
Whakaritenga
| Nga Tuemi Whakamatau | Whakatakotoranga | Hua |
| (SDS PAGE) Pure o te Hoa Enzyme(SDS PAGE) | ≥95% | Paahi |
| Mahi Endonuclease | Kaore i kitea | Paahi |
| Mahi Exodulease | Kaore i kitea | Paahi |
| Mahi Rnase | Kaore i kitea | Paahi |
| Te toenga E.coli DNA | ＜1 kape/60 | Paahi |
| Pūnaha Whakamātautau Mahi | 90%≤110% | Paahi |
Waehanga
| Waehanga | 100rxns | 1,000rns | 5,000 rxns |
| RNase-kore ddH2O | 2*1ml | 20ml | 100ml |
| 5 * kotahi te ranunga taahiraa | 600μl | 6*1ml | 30ml |
| Kotahi te hikoi te ranunga enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX tohutoro Waikano 1 | 60μl | 600μl | 3*1ml |
| 50* ROX tohutoro Waikano 2 | 60μl | 600μl | 3*1ml |
a.Kotahi-Step Buffer kei roto te dNTP Mix me te Mg2+.
b.Ko te Rahui Enzyme te nuinga he whakamuri
transcriptase, Hot Start Taq DNA polymerase (whakarereke antibody) me te aukati RNase.
c.Ka whakamahia ki te whakatika i te hapa o nga hihi rewharewha i waenga i nga puna rereke.
d.ROX: Me whiriwhiri koe i te whakatikatika kia rite ki te tauira o te taputapu whakamatautau.
Nga tono
Te kitenga QPCR
Te tuku me te rokiroki
Tukunga:Puka tio
Nga Tikanga Rokiroki:Rokiroki ki te -20 ℃.
Shief Life:18marama
