Nuclease Ultra
Ko te UltraNuclease he ira ira e tendonuclaese i ahu mai i te Serratia marcescens, e kaha ana ki te whakaheke i te DNA, i te RNA ranei, he paerua, he pae kotahi ranei, he raina, he porohita ranei i raro i te whānuitanga o nga ahuatanga, ka tino whakaheke i nga waikawa karihi ki te 5'-monophosphate oligonucleotides me te 3-5base te roa. .I muri i te whakarereketanga o te hangarau ira, ka fermented te hua, ka whakahuahia, ka purea ki te Escherichia coli (E. coli), e whakaiti ana i te kaha o te supernatant pūtau me te rangahau lysate pūtau, engari ka pai ake te purenga me te rangahau mahi o te pūmua.Ka taea hoki te whakamahi i roto i te rongoa ira, te purenga huaketo, te hanga kano kano, te pūmua me te ahumahi rongoa polysaccharide hei reagent tango waikawa nucleic reagent.
āhuatanga hua
| CAS No. | 9025-65-4 |
| EC No. | |
| Taumaha Molecular | 30kDa |
| Ikohikohiko | 6.85 |
| Pūmua Pure | ≥99%（ SDS-WAHARANGA me SEC-HPLC） |
| Mahi Tauwhāiti | ≥1.1×106U/mg |
| Temperature tino pai | 37°C |
| pH tino pai | 8.0 |
| Te Mahi Porotei | kino |
| Koiora | ＜10CFU/100,000U |
| Pūmua Pūtau-Kaihautū toenga | ≤10ppm |
| Kounga Taumaha | ≤10ppm |
| Endotoxin huakita | ＜0.25EU/1000U |
| Pupuri Rokiroki | 20mM Tris-HCl, pH 8.0, 2mM MgCl2 ， 20mM NaCl ，50% Glycerol |
Nga tikanga rokiroki
≤0°C te kawe;-25~-15°C Rokiroki，2 tau te mana (kaore i te tio-whakarewa).
Whakamaramatanga Waeine
Ko te nui o te whākōkī i whakamahia ki te huri i te uara absorption o △A260 mā te 1.0 i roto i te 30min i te 37 °C, pH 8.0, he rite ki te 37μg salmon sperm DNA kua keria ma te tapahi ki te oligonucleotides, i tautuhia hei waeine kaha(U).
Mana kounga
Pūmua Pūtau-Kaihautū toenga: kete ELISA
•Protease Nga toenga: 250KU/mL UltraNuclease tauhohe ki tïpako mo 60min, kahore mahi i kitea.
•Endotoxin huakita: LAL-Test, Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition) te tikanga whakamatautau here.Nga Ture Nui (1143).
•Koiora: Pharmacopoeia of the People's Republic of China Volume 4 (2020 Edition)— General
Nga Ture mo te Whakamatau Whakamatau (1101), PRC National Standard, GB 4789.2-2016.
•Metal Taumaha:ICP-AES, HJ776-2015.
Mahi
Ko te mahi UltraNuclease i tino whakakorehia i te wa i neke atu te kukū o te SDS i te 0.1%, i te EDTA ranei
Neke atu i te 1mM te kukū. Ko te Surfactant Triton X- 100, Tween 20 me Tween 80 kaore he paanga ki te karihi.
āhuatanga i te wa i raro te kukū i te 1.5%.
| Mahi | Mahi tino pai | Mahi Whaimana |
| Te pāmahana | 37℃ | 0-45 ℃ |
| pH | 8.0-9.2 | 6.0- 11.0 |
| Mg2+ | 1-2mM | 1- 15mM |
| DTT | 0- 100mM | >100mM |
| 2-Mercaptoethanol | 0- 100mM | >100mM |
| Katote whakarewa monovalent (Na+, K+ etc.) | 0-20mM | 0-200mM |
| PO43- | 0- 10mM | 0- 100mM |
Te Whakamahi me te Inenga
• Tangohia te waikawa karihi o waho mai i nga hua kano kano, whakaitihia te mate o te toenga o te paitini waikawa karihi me te whakapai ake i te haumaru hua.
• Whakaitihia te pokey o te wai whangai na te waikawa karihi, whakapoto i te wa tukatuka me te whakanui ake i te hua o te pūmua.
• Tangohia te waikawa nucleic i takai matūriki (huaketo, tinana whakauru, aha atu), he pai
ki te tuku me te purenga o te matūriki.
| Momo Whakamatau | Hanga Pūmua | Virus, Kano kano | Rongoa Pūtau |
| Tau Pūtau | 1g pūtau taumaha mākū (kua whakatārewatia me te 10ml pūreirei) | 1L FERMENTATION wē supernatant | 1L tikanga |
| Inenga iti | 250U | 100U | 100U |
| Inenga Manakohia | 2500U | 25000U | 5000U |
• Ka taea e te maimoatanga karihi te whakapai ake i te whakatau me te whakaora i te tauira mo te chromatography tīwae, electrophoresis me te tātari blotting.
• I roto i te whakamaarama ira, ka tangohia te waikawa karihi ki te whiwhi i nga huaketo adeno-hononga.
