RT-LAMP Colormetric Master Mix HCB5204A
Kei roto i tenei hua he parepare tauhohenga, RT-Enzymes Mix (Bst DNA polymerase me te wera-aukati whakamuri transcriptase), lyophilized Protectants me nga waahanga waikano chromogenic.Hei whakamahi, me whakamahi noa i te Buffer, ka konatunatua te enzyme tauhohenga me te tuatahi ka honoa ki te tauira;Ko te taapiri i te whakamarumaru lyophilized ka taea te tika.I honoa ki te lyophilizer me te lyophilized, a ko nga mea tuatahi me nga tauira i taapiri ina whakamahia ana.Ko tenei kete he tere, he maamaa te kitenga o te whakakaha, ko te tauhohenga kino e tohuhia ana i te whero me te tauhohenga pai ka tohuhia e te huringa ki te kowhai.
Waehanga
| Waehanga | HCB5204A-01 | HCB5204A-02 | HCB5204A-03 |
| Kaipupuri Whakanui-whakawaenga-a-roopu (me te tae) | 0.96 mL | 4.80 mL×2 | 9.60 mL×10 |
| RT-Enzymes Mix | 270 μL | 2.70 mL | 2.70 mL×10 |
| He parenga Lyophilized | 0.96 mL×2 | 9.60 mL×2 | 9.60 mL×20 |
Nga tono
Mo te DNA, te RNA ranei te whakanui i te hauhauariki.
Nga Tikanga Rokiroki
Ka haria me te hukapapa maroke, ka rongoa ki te -25~ -15 ℃.A ape i te tio-whakarewa, he mana te hua mo te 12 marama.
Kawa
1.Whakarewa i te parepare tauhohenga hei whakamahi i te pāmahana rūma.He wa poto ka huri, ka hurihia ranei nga ngongo kia pai te uru, katahi ka whakahiatohia kia kohia te wai ki raro o te ngongo.
2.Te whakarite i te punaha tauhohenga.Ka taea te whakarite i tenei reagent i roto i nga punaha tauhohenga e rua, te ranunga tauhohenga wai me te ranunga punaha lyophilized.
1) Whakaritea te ranunga tauhohenga wai
| Waehanga | Rōrahi |
| Kaipupuri Whakanui-whakawaenga-a-roopu (me te tae) | 10 μL |
| RT-Enzymes Mix | 2.8 μL |
| 10 × Whakaranu Tuatahia | 5 μL |
| Nga tauira DNA/RNA b | × μL |
| Wai kore-nuclease | Tae atu ki te 50 μL |
2) Rahui pūnaha Lyophilization
① Whakaritea te ranunga lyophilized
| Waehanga | Rōrahi |
| Kaipupuri Whakanui-whakawaenga-a-roopu (me te tae) | 10 μL |
| He parenga Lyophilized | 20 μL |
| RT-Enzymes Mix | 2.8 μL |
| Wai kore-nuclease | Tae atu ki te 50 μL |
② Lyophilization: Ko te Mix kua oti te whakarite i lyophilized i roto i te punaha 50μL
③ Whakaritea te ranunga tauhohenga
| Waehanga | Rōrahi |
| Te ranunga lyophilized | 1 wahi |
| 10 × Whakaranu Tuatahia | 5 μL |
| Nga tauira DNA/RNA b | × μL |
| Wai kore-nuclease | Tae atu ki te 50 μL |
Tuhipoka:
1) a.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b.E taunaki ana te DEPC (wairewa wai) mo te templ waikawa karihi.
1.Whakanuia ki te 65°C mo te 30-45mins, ka taea te whakaroa tika kia rite ki te huringa tae Te wa tauhohenga.
2.E ai ki te kanohi tahanga, he pai te kowhai, he kino te whero.
Notes
1.Ka puta pea te tote ki raro o te ngongo parepare, ka poipoipoi mo te wa poto, ka huri ranei nga ngongo kia maha nga wa ka uru pai ki te pāmahana rūma.
2.Ka taea te whakapai ake i te pāmahana tauhohenga i waenga i te 62 ℃ me te 68 ℃ i runga i te ahua o nga timatanga.
3.Ko nga reagents kua oti te takai kia kaua e noho ki te hau mo te wa roa.
4.Ko te tauhohenga whakaheke whero me te kowhai ka whakawhirinaki ki te huringa pH o te punaha tauhohenga, kaua e whakamahi i te otinga rokiroki waikawa nucleic Tris kei roto, e taunaki ana kia whakamahia te ddH2E te waikawa karihi rongoa;
5.Ko te whakamatautau me whakataurite, tae atu ki te whakarite i te punaha tauhohenga, te lyophilization, me te tukatuka tauira me te tauira taapiri;
6.Hei karo i te poke, e taunaki ana kia whakareri i te punaha tauhohenga ki roto i te papaa-ma-ma-ma, i etahi atu Taapiri tauira ki te kohu kohu o te ruma kia kore ai e pokanoa te pai.
