RT-LAMP Fluorescent (Lyophilized ball)
Ko te RT-LAMP Fluorescent Master Mix (Lyophilized Powder) kei roto he parepare tauhohenga, Bst DNA polymerase me te whakamuri transcriptase whakamuri-wera, te whakamarumaru lyophilized, me te waikano rewharewha.Ko te hua kei roto i te ahua o nga pirepire lyophilized, a ko nga mea tuatahi me nga tauira me taapiri hei whakamahi.
Wae Reagent
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Whakamahinga
Mo te DNA, te RNA ranei te whakanui i te hauhauariki
Nga Tikanga Rokiroki
Te kawe me te rokiroki i te 2~8°C.
Te ora o te papa: 12 marama.
Kawa
1. Tangohia te maha o nga pirepire lyophilized i runga i te maha o nga whakamatautau.
2. Te whakarite pūnaha tauhohenga
| Waehanga | Inenga |
| RT-LAMP Fluorescent Master Mix (Lyophilized Powder) | 2 pirepire |
| Waikano Korewharewha | x μL |
| 10 × Primer Mix a | 5 μL |
| Nga tauira DNA/ RNA b | n μL |
| Tapeke | 50 μL |
Tuhipoka:
1) a.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b.E taunaki ana te tauira waikawa karihi kia memeha ki te wai DEPC.
Hyasen Biotechnology Co., Ltd
3) Hotaka tauhohenga
| Te pāmahana | Te wa | Nga huringa |
| 65℃ | 60s a | 30 |
Tuhipoka:
1. Kohia te tohu kareoro
Notes
1. Ka taea te whakapai ake i te pāmahana tauhohenga i waenga i te 62°C me te 68°C e ai ki te tuatahitikanga;
2. Ko te whakamatautau me whakataurite i te mahi, tae atu ki te whakarite i te punaha tauhohenga,te tukatuka tauira me te horopeta;
3. Ki te karo contamination, e tūtohu ana ki te whakarite i te pūnaha tauhohenga i roto i te tepu ultra-mame te taapiri i nga tauira ki te kohu kohuke i etahi atu ruma kia kore ai e pokanoa te pai.
