Glycerol Kinase(GK)
Whakaahuatanga
Ko te pūmua i whakawaeheretia e tenei ira no te whanau kinase FGGY.Ko tenei pūmua he whākōkī matua i roto i te whakaritenga o te glycerol uptake me te pākia.Ka whakakorikori i te phosphorylation o te glycerol na te ATP, te tuku ADP me te glycerol-3-phosphate.Ko nga huringa i roto i tenei ira e hono ana ki te ngoikoretanga o te glycerol kinase (GKD).Kua kitea nga momo momo tuhi kua honoa mo tenei ira.
Ka whakamahia tenei enzyme mo nga whakamatautau whakamatautau mo te whakatau i nga triglycerides me te Glycerol-3-phosphate Oxidase.
Hanganga matū
Kaupapa Tauhohenga
Glycerol + ATP→ Glycerol -3- phosphate + ADP
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | He ma ki te paura amorphous paku kowhai, kua lyophilized |
| Mahi | ≥15U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa (10mg paura/ml) | Maamaa |
| Catalase | ≤0.001% |
| Te huka oxidase | ≤0.01% |
| Uricase | ≤0.01% |
| ATPase | ≤0.005% |
| Hexokinase | ≤0.01% |
Te kawe me te rokiroki
Te kawe waka:Ka tukuna i raro i te -15°C
Rokiroki :Rokiroki ki te -20°C(Wā roa), 2-8°C(Wa poto)
Ka tūtohuhia te whakamatautau anoTe ora:18 marama
hua e pa ana
Tuhia to korero ki konei ka tukuna mai ki a matou
