Bst 2.0 DNA Polymerase (Glycerol free, kiato teitei)
Ko te Bst DNA polymerase V2 i ahu mai i te Bacillus stearothermophilus DNA Polymerase I, he 5′→3′ te mahi DNA polymerase me te mahi whakakapi mekameka kaha, engari kaore he mahi 5′→3′ exonuclease.Ko te Bst DNA Polymerase V2 he mea pai mo te whenu-displacement, te whakakaha hauwaro i te RAMA (Loop mediated isothermal amplification) me te raupapa tere.Ko tenei Bst DNA polymerase V2 he kaha ki te aukati i te mahi DNA polymerase i te pāmahana rūma, kia taea ai te whakahaere me te punaha tauhohenga ka taea te hanga i te pāmahana rūma, te aukati i te whakakaha kore-motuhake me te whakapai ake i te pai o te tauhohenga, a ka taea e tenei putanga. kia lyophilized.I tua atu, ka taea e ia te tuku i tana mahi i te teitei o te mahana, na reira ka whakakorehia te hiahia mo te waahanga whakahohe motuhake.
Waehanga
| Waehanga | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-free) (32U/μL) | 0.05 mL | 0.25 mL | 2.5 mL |
| 10×HC Bst V2 Kaipupuri | 1.5 mL | 2×1.5 mL | 3×10 mL |
| MgSO4 (100mM) | 1.5 mL | 2×1.5 mL | 2×10 mL |
Nga tono
1.Ko te whakakaha hau werawera LAMP
2.Tauhohenga nuku kotahi te whenu DNA
3.Te raupapa ira GC teitei
4.Te raupapa DNA o te taumata nanogram.
Tikanga Rokiroki
Te kawe i raro i te 0°C ka penapena ki te -25°C~-15°C.
Whakamaramatanga Waeine
Kotahi te waeine kua tautuhia ko te nui o te whākōkī e whakauru ana i te 25 nmol o te dNTP ki roto i te waikawa waikawa i roto i te 30 meneti i te 65°C.
Tauhohenga RAMA
| Waehanga | 25 μLPūnaha |
| 10×HC Bst V2 Kaipupuri | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTP (10mM ia) | 3.5 μL |
| SYTO™ 16 Kākāriki (25×)a | 1.0 μL |
| Te ranunga tuatahib | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-free) (32 U/uL) | 0.25 μL |
| tauira | × μL |
| ddH₂O | Tae atu ki te 25 μL |
Tuhipoka:
1) a.SYTOTM 16 Kākāriki (25×): E ai ki nga hiahia whakamatautau, ka taea te whakamahi etahi atu waikano hei whakakapi;
2) b.Te ranunga tuatahi: ka whiwhi ma te ranu 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB me etahi atu pukapuka.
Tauhohenga me te Tikanga
1 × HC Bst V2 Buffer, kei waenganui i te 60°C me te 65°C te pāmahana heki.
Te Wera Whakakore
80°C, 20 meneti.
