RT-LAMP Colormetric (Poro Lyophilized)

Kei roto i tenei hua he parepare tauhohenga, RT-Enzymes Mix (Bst DNA polymerase me te wera-aukati whakamuri transcriptase), he whakamarumaru lyophilized me nga waahanga waikano chromogenic.Ko te hua he momo poipoi lyophilized, ma te whakamahi noa me nga tauira me nga tauira.Ko tenei kete he tere, he maamaa te kitenga o te whakakaha, ko te tauhohenga kino e tohuhia ana i te whero me te tauhohenga pai ka tohuhia e te huringa ki te kowhai.

Waehanga

RT-LAMP Colormetric Master Mix (Nga pirepire Lyophilized)

Nga tono

Mo te DNA, te RNA ranei te whakanui i te hauhauariki.

Nga Tikanga Rokiroki

Ka kawea, ka rongoa ki te 2~ 8 ℃.He mana te hua mo te 12 marama.

Kawa

1.Tangohia te tau e rite ana Lyophilized beads paura i runga i te maha o nga whakamatautau.

2.Whakaritea te ranunga tauhohenga

Tuhipoka:

1. 10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

2. Ko nga tauira waikawa nucleic e taunaki ana kia memeha ma te wai DEPC.

3.Whakanuia ki te 65°C mo te 30-45mins, ka taea te whakaroa tika kia rite ki te huringa tae Te wa tauhohenga.

4.E ai ki te kanohi tahanga, he pai te kowhai, he kino te whero.

Notes

1.Ka taea te whakatika i te pāmahana tauhohenga i waenga i te 62 ℃ me te 68 ℃ i runga i te ahua tuatahi.

2.Ko nga reagents kua oti te takai kia kaua e noho ki te hau mo te wa roa.

3.Ko te tauhohenga whakaheke whero me te kowhai e whakawhirinaki ana ki te huringa pH o te punaha tauhohenga, kaua e whakamahi i te otinga rokiroki waikawa karihi Tris kei roto, e taunaki ana kia whakamahia te ddH₂O te waikawa nucleic rongoa.

4.Ko te whakamatautau me whakahaere i runga i nga tikanga paerewa, tae atu ki te whakarite i te punaha tauhohenga, te maimoatanga tauira me te taapiri tauira.

5.E whakaarotia ana kia whakareri i te punaha tauhohenga i roto i te teepu ultra-ma me te taapiri i nga tauira ki roto i te kohu kohu o etahi atu ruma kia kore ai e teka.