Rnase Inhibitor (Glycerol free)
Murine RNase inhibitor Ko te recombinant murine RNase inhibitor whakapuakihia, ka purea i E.coli.Ka herea ki te RNase A, B, C ranei i roto i te 1:1 ōwehenga na roto i te hononga kore-covalent, na reira ka aukati i te mahi o nga enzymes e toru me te tiaki i te RNA mai i te paheketanga.Engari, Kaore e whai hua ki te RNase 1, RNase T1, S1 Nuclease, RNase H, RNase ranei mai i Aspergillus.I whakamatauria te aukati Murine RNase e te RT-PCR, RT-qPCR me te IVT mRNA, a he hototahi ki nga momo whakawhitinga whakamuri arumoni, DNA polymerases me RNA polymerases.
Ki te whakatauritea ki nga kaitautoko RNase tangata, karekau he rua cysteinees e uru ana te aukati RNase murine e tino aro nui ana ki te waiora e puta ai te koretake o te aukati.Ka noho pumau ki te iti o te DTT (iti iho i te 1 mM).Ko tenei ahuatanga e pai ana mo te whakamahi i nga tauhohenga ka nui te kino o te DTT ki te tauhohenga (hei tauira, RT-PCR-Wa-Tuturu).
Application
Ka taea te whakamahi whanui i tenei hua i roto i nga whakamatautau ka taea te wawaotanga RNase ki te karo i te paheketanga o te RNA, penei:
1.Tuatahi-wau cDNA synthesis, RT-PCR, RT-qPCR, etc.;
2. Tiakina te RNA mai i te paheketanga i roto i te whakamaoritanga in-vitro/whakamaoritanga (hei tauira, te tukurua viral in vitro);
3.Ko te whakakore i te mahi RNase i te wa e wehe ana te RNA me te purenga.
Nga Tikanga Rokiroki
Rokiroki ki te -25～-15℃;
Nga huringa whakatio-rewa ≤ 5 nga wa;
Mana mo te 1 tau.
Te whakamaramatanga o te waeine
Ko tetahi waahanga kua tautuhia ko te nui o te RNase Inhibitor e hiahiatia ana hei aukati i te mahi o te 5 ng o RNase A e 50%.
Te taumaha ngota
RNase Inhibitor (Glycerol-free) he pūmua 50 kDa.
Mana kounga
Exonuclease Mahi:
Ko te whakauru o te 40 U o te enzyme me te 1 μg λ-Hind III digest DNA mo nga haora 16 i te 37°C karekau e kitea te whakahekenga o te DNA i whakatauhia e te electrophoresis gel.
Mahi Endonuclease:
Ko te whakauru o te 40 U o te enzyme me te 1 μg λ DNA mo te 16 haora i te 37°C ka kore e kitea te kino o te DNA i whakatauhia e te electrophoresis gel.
Te tima Mahi:
Ko te whakamautanga o te 40 U o te whākōkī me te 1 μg pBR322 mo te 16 haora i te 37 ℃ karekau e kitea te kino o te DNA i whakatauhia e te electrophoresis gel.
RNase Mahi:
Ko te whakamautanga o te 40 U o te whākōkī me te 1.6 μg MS2 RNA mō te 4 hāora i te 37 ℃ karekau e kitea te paheketanga o te RNA i whakatauhia e te electrophoresis gel.
E.coli DNA:
40 U o Enzyme ka kitea e TaqMan qPCR.Ko te DNA E.coli he ≤ 0. 1pg/40U.
Notes
1.Kaua e ruru, e ohooho kaha ranei kia kore ai e whakakorikori te enzyme.
Kei te kaha te aukati 2.RNase i nga mahana mai i te 25 ℃ ki te 55 ℃, ka kore e taea te ≥65 ℃.
3.Ko te mahi a RNase H, RNase 1, me RNase T1 kaore i te whakakorehia.
4.Ko te awhe pH mo te aukati i te mahi RNase he whanui (he kaha ki te pH 5-9), e whakaatu ana i nga mahi teitei i te pH 7-8.
