Hexokinase (HK)
Whakaahuatanga
Whakamahia te Hexokinase mo te whakatau i te D-glucose, D-fructose, me te D-sorbitol i roto i nga tauira rangahau kai, koiora ranei.Ka whakamahia ano te enamaki mo te whakamatautau i etahi atu saccharides ka taea te huri ki te hukahuka, ki te fructose ranei, na reira ka whai hua ki te whakamatautau i te maha o nga glycosides.
Mena ka whakamahia te Hexokinase me te glucose-6-phosphate dehydrogenase (G6P-DH)* (te whakamatautau i te glucose6-phosphate i hangaia e te Hexokinase), kaua nga tauira i te nui o te konupora phosphate i te mea ko te G6P-DH te whakataetae e aukatihia ana e te phosphate.
Hanganga matū
Kaupapa Tauhohenga
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Whakatakotoranga
| Nga Tuemi Whakamatau | Whakatakotoranga |
| Whakaahuatanga | He ma ki te kowhai iti paura amorphous, lyophilized |
| Mahi | ≥30U/mg |
| Pure(SDS-PAGE) | ≥90% |
| Wairewa (10mg paura/ml) | Maamaa |
| Porotei | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Te kawe me te rokiroki
Te kawe waka: Ambient
Rokiroki :Rokiroki ki te -20°C(Waa roa), 2-8°C (waa poto)
Ka tūtohuhia te whakamatautau anoTe ora:1 tau
