DNase I (Rnase Koreutu) (2u/ul)
Cat Nama: HC4007B
Ko te DNase I he endonuclease ka taea te keri i te DNA whenu kotahi-rua ranei.Ka taea e ia te whakamaarama i nga here phosphodiester ki te whakaputa i te mono-me te oligodeoxynucleotides kei roto he roopu 5'-phosphate me te roopu 3'-OH.Ko te awhe pH mahi tino pai o DNase I ko 7-8.Ko te mahi a DNase I ka whakawhirinaki ki te Ca2+a ka taea te whakahohe e nga katote whakarewa divalent penei i te CO2, Mn2+, Zn2+, etc I te aroaro o Mg2+, DNase Ka taea e au te wehe matapōkeretia tetahi pae o te DNA paerua;I te aroaro o Mn2+, DNase Ka taea e au te wetewete i te DNA ki te pae-rua ki te waahi kotahi, ka hanga i nga pito puhuki, i nga pito piri me te 1-2 nucleotides e puta ana.Ka taea te whakamahi mo te tukatuka o nga momo tauira RNA.
Waehanga
| Ingoa | 1KU | 5KU |
| DNaseI Recombinant (RNase-kore) | 500 μL | 5 ×500 μL |
| DNase I Reaction Buffer (10×) | 1 mL | 5 × 1mL |
Nga tikanga rokiroki
Me penapena tenei hua ki te -25~-15 ℃ mo te 2 tau.Tena koa karohia te whakamao-whakatio.
Tohutohu
I whakamahia ki te tango i te DNA mai i nga tauira RNA hei tohutoro anake.
1. Tena koa whakamahia nga ngongo centrifuge RNase-kore me nga tohutohu paipa hei whakarite i te punaha tauhohenga e whai ake nei:
| Waehanga | Rōrahi (μL) |
| DNase I Reaction Buffer (10×) | 1 |
| DNasel Recombinant (RNase-kore) | 1 |
| RNA | X |
| RNase-kore ddH2O | Tae atu ki te 10 |
2. Ko nga tikanga tauhohenga e whai ake nei: 37 ℃, i muri i te 15-30 meneti, tāpirihia te kukū whakamutunga o te otinga 2.5 mM EDTA ka uru pai, ka 65 ℃ mo te 10 meneti.Ka taea te whakamahi i te tauira kua tukatukahia mo nga whakamatautau RT-PCR, RT-qPCR ranei, me era atu.
Notes
1. He tairongo a DNase l ki te denaturation tinana;I te wa e ranu ana, whakahurihia te ipuipu whakamatautau me tekia pai te wiri, kaua e wiri kaha.
2. Me penapena te enzyme ki roto i te pouaka tio, ki runga ranei i te pati tio ina whakamahia, me rongoa ki te -20 ℃ i muri tonu i te whakamahinga.
3. Ko tenei hua mo te rangahau anake.
4. Tena koa mahi me nga koti taiwhanga me nga karapu tuku, mo to haumaru.
