Hoamata Taq DNA Polymerase (5u/ul)
Ko te Taq DNA Polymerase he DNA polymerase timatanga wera me te aukati rua e nga antibodies rua. Ko tenei hua kaore i te aukati noa i te mahi polymerase 5′→3′ o Taq DNA polymerase, engari ka aukati ano i te mahi 5′→3′exonuclease.Ko te whakamahana mo te 30 hēkona i te pāmahana i mua i te whakahekenga ka taea te whakakore i te antibody me te tuku i te mahi polymerase DNA me te mahi exonuclease.Ko nga ahuatanga aukati rua e kore e taea anake te aukati i te whakakaha kore-motuhake i puta mai i te koretake, i te dimer tuatahi ranei, engari he pai te aukati i te heke o te tohu fluorescence na te paheketanga o te tirotiro, kia pai ake ai te noho o te reagent in vitro i te wa e kawe ana, e whakamahi ana ranei i te ruuma. pāmahana.
Waehanga
| Waehanga | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μL | 200 μL | 2 mL | 5 mL |
Tikanga Rokiroki
Ka tukuna te hua ki te hukapapa maroke ka taea te rongoa ki te -25°C~-15°C mo te 2 tau.
Whakatakotoranga
| Polymerase | Taq DNA Polymerase |
| Maamaa | ≥ 95% (SDS-WĀRANGI) |
| Tīmata wera | Hanga-i roto i te Hot Start |
| Tere Hohenga | Paerewa |
| Mahi Exonuclease | 5′→3′ |
Tohutohu
Tauhohenga Tatūnga
| Waehanga | Rōrahi (μL) | Te Whakamutunga Whakamutunga |
| 2× Kaipupuria | 25 | 1× |
| Te ranunga o te Primer/Probeb | × | 0.1 μmol/L-0.5 μmol/L |
| Wehenga Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Tātauira DNAc | × | 0.1-100 ng |
| ddH2O | Tae atu ki te 50 | - |
Tuhipoka:
1) E ai ki te tono whakamätautau motuhake, me whakareri i te parepare tauhohenga.
2) Ko te nui o te DNA me te kukū o nga rangahau, i nga mea tuatahi ranei e taunaki ana.Ko te kukū tino pai ka taea te whakarite kia rite ki nga ahuatanga whakamatautau motuhake.
Kawa paihikara wera
| Hipanga | Te pāmahana(°C) | Te wa | Nga huringa |
| Tuhinga o mua | 95 ℃ | 5min | 1 |
| Te whakahekenga | 95 ℃ | 15 hēkona | 45 |
| Whakawhana / Toronga | 60 ℃a | 30 hēkonab |
Tuhipoka:
1) Ka whakatikahia te pāmahana tauhohenga kia rite ki te uara Tm o nga kaupapa tuatahi i hangaia.
2) Ko nga taputapu qPCR rereke e hiahia ana kia rereke te waa whiwhi tohu fluorescence, me whakarite kia rite ki te waa poto rawa atu.
Notes
Tena koa mauhia nga PPE e tika ana, he koti taiwhanga me nga karapu, kia pai ai to hauora me to haumaru!
